Transcriptional Profiling and Investigation of Expression-Guided Feeding Strategies for Bioprocess Development

The BIOSCALE team aims to systematize scale-up in biotechnological processes, thereby enabling reduced development times, higher success rates, and improved process quality for scale-up procedures.

My work focuses on characterizing feeding strategies through transcriptomic analysis, combining next-generation RNA sequencing with biological readouts. Since fed-batch cultivation is a dominant strategy in industrial biotechnology, I investigate how cells adapt to nutrient limitation or excess and how these adaptations affect productivity and stress responses. By systematically linking feeding strategies to gene expression profiles, regulatory networks, and reporter signals, my goal is to advance biologically driven feeding regimes that enhance process robustness and efficiency. This systematic approach aims to improve bioprocess scalability while aligning it with microbial physiology.

• 10/2017 - 08/2021 Bachelor of Science; Bioengineering; Karlsruhe Institute of Technology (KIT)
• 12/2020 - 04/2021 Bachelor Thesis: Cellulolytic and hemicellulolytic abilities of Parageobacillus thermoglucosidasius; Institute of Process Engineering in Life Sciences, Technical biology, Karlsruhe Institute of Technology (KIT)
• 10/2021 – 04/24 Master of Science; Bioengineering; Karlsruhe Institute of Technology (KIT)
• 08/2023 – 12/2023 Internship: Formulation and cryopreservation of stem cells; Bayer AG, AMES – Drug Substance Technologies – Cell Therapy, Leverkusen
• 08/2024 – 01/2025 Master Thesis: Establishment of a downstream process strategy for carotenoid products and the production of a carotenoid-containing formulation for the cosmetics industry; Institute of Process Engineering in Life Sciences, Microsystems in Bioprocess Engineering, “BIOSCALE” research group
• 05/2025 – present PhD candidate; Institute of Process Engineering in Life Sciences, Microsystems in Bioprocess Engineering, “BIOSCALE” research group